The difference is...

Date: 2008-02-23 10:59 pm (UTC)
"Wounded oysters build out of gory wounds a pearl And create within the gap of pain a pearl”

As far as I can tell, the difference between freshwater & saltwater is that freshwater are cultured by the introduction of a piece of mantle tissue & saltwater are cultured by the introduction of mantle tissue and a mother of pearl bead. This implies that freshwater are higher quality because they are nacre all the way through.

Some sights I've been to say that all pearls are 'cultured' these days, which means they wouldn't exist without the intervention of humans.
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